Introduction

The diamondback moth (DBM), Plutella xylostella, (Lepidoptera: Plutellidae) is one of the most important insect pests, preferentially feeding on cruciferous plants. DBM damage has been recorded in every country producing crucifers, and it is thought to be the world's most widespread lepidopteran insects. DBM management remains a challenge because of its relatively short life cycle, high reproductive rate, wide host range of cruciferous plants and rapid evolution of insecticide resistance. Genome sequencing has become an essential step for unveiling the molecular interactions between the DBM and its host plant crucifers, and is important for understanding of insecticide resistance evolution and its management.

We sequenced the DBM genome using fosmid clones (~10X of the genome size) and the whole genome shotgun approach (~213X) using the Illumina sequencing platform. We developed custom software for assembling heterozygous data. The resulting 343 Mb genome (consisting of 1,819 scaffolds, with an N50 of 737 kb) contains 18,071 protein-coding genes, 33.97% for the repetitive sequences and 781 non-coding RNAs. The assembly covered 85.5% of a set of protein-coding expressed sequence tags (ESTs) generated by transcriptome sequencing.

To provide public access to the genomic resources of the DBM, we have developed a database of the DBM genome (DBM-DB) based on the completed genome sequence, which can be found at http://iae.fafu.edu.cn/DBM. The information from the DBM-DB can be useful for further research on functional genomics because we also provide the data from RNA-seq for transcriptomic analysis and digital gene expression profiling(coming soon). The data for microRNAs and metagenomics in the DBM midgut are coming soon.

Addition

To better service the users of diamondback moth database, we provide following two websites for visiting DBM-DB:

1) DBM-DB Mirror Site 1: http://iae.fafu.edu.cn/DBM.

2) DBM-DB Mirror Site 2: http://59.79.254.1/DBM.